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Comment: Heatmap of rnaseq data with z-score scale
by
Paul Harrison
▴ 110
There can be considerable differences between datasets just due to different handling, sequencing method, batch effects, etc etc, and this …
Comment: Memory issue while using "quickcluster" and "computeSumFactors" functions on sin
by
ATpoint
★ 4.8k
What is lac cells? You mean 7000-9000 cells? Please check first that the function runs OOM even without any of these multicore options set.…
Comment: parameters of filterByExpr
by
caroline.zanchi
▴ 10
It worked, I think I finally understand and accept that fact. Sorry and thank you again !
Comment: parameters of filterByExpr
by
Gordon Smyth
52k
You seem to be coming back to the same fixed (but incorrect) idea of what filterByExpr() should be doing. I have tried to explain that filt…
Answer: edgeR in DMR mode - replicates give batch effect - how to build design matrix wi
by
Gordon Smyth
52k
modelMatrixMeth() works correctly. The second replicate factor has not been lost. Please do not make ad hoc manual modifications to the de…
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Comment: Heatmap of rnaseq data with z-score scale
Answer: parameters of filterByExpr
Comment: Heatmap of rnaseq data with z-score scale
Answer: LogFC values in edgeR - w/wo intercept
How to formulate 2 factor nested design in DESeq2
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